Improved Membrane Filter Method for Fecal Coliform Analysis
نویسندگان
چکیده
منابع مشابه
Improved method for coliform verification.
Modification of a method for coliform verification presented in Standard Methods for the Examination of Water and Wastewater is described. Modification of the method, which is based on beta-galactosidase production, involves incorporation of a lactose operon inducer in medium upon which presumptive coliform isolates are cultured prior to beta-galactosidase assay.
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In vitro pure culture studies were conducted on three different strains of Escherichia coli (K-12, EPA 00244, and SWEI) to determine the effect of chlorination on catalase activity. In each case, stationary-phase cells exhibited significant (P less than 0.001) reductions in enzyme activity following exposure to chlorine. Mean differences in activity between control and chlorine-stressed cells r...
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The standard one-step M-FC broth-membrane-filter procedure for recovery of fecal coliforms from chlorinated sewage effluents is much less effective than the multiple-tube (most-probable-number) technique. A two-step membrane-filter method, using a pre-enrichment technique with phenol red lactose broth and incubation at 35 degrees C for 4 h, followether 18+/-2 h, enhanced fecal coliform recovery...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1975
ISSN: 0003-6919
DOI: 10.1128/am.29.4.532-536.1975